E02M1060 Rat Marinobufagenin ELISA kit
The Rat Marinobufagenin ELISA kit can be used to identify samples from the rat species. Marinobufagenin can also be called MBG.
Specifications of Rat Marinobufagenin ELISA kit
Product Information | |
Cat. No. | E02M1060 |
Product Name | Rat Marinobufagenin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 nmol/L |
Sensitivity | 0.1 nmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 nmol/L | 1 vial |
STANDARD B (0.5mL) | 0.5 nmol/L | 1 vial |
STANDARD C (0.5mL) | 1.0 nmol/L | 1 vial |
STANDARD D (0.5mL) | 2.5 nmol/L | 1 vial |
STANDARD E (0.5mL) | 5.0 nmol/L | 1 vial |
STANDARD F (0.5mL) | 10 nmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MBG ELISA kit uses an anti-MBG antibody and an MBG-HRP conjugate in a competitive enzyme immunoassay method. MBG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MBG antibody binding site between MBG from samples and MBG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MBG. Since the number of sites is limited, as more sites are occupied by MBG from the sample, fewer sites are left to bind MBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBG concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Marinobufagenin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MBG and analogues was observed. | |
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Summary of the Assay Procedure for Rat Marinobufagenin ELISA kit
Citations of Rat Marinobufagenin ELISA kit
E02M1060 has been referenced in the below publications：
Model of Chronic Thromboembolic Pulmonary Hypertension in Rats Caused by Repeated Intravenous Administration of Partially Biodegradable Sodium Alginate Microspheres.
