E02M0302 Rat Matrix metalloproteinase 13 ELISA kit
The Rat Matrix metalloproteinase 13 ELISA kit can be used to identify samples from the rat species. Matrix metalloproteinase 13 can also be called CLG3, Collagenase 3, MMP-13, MMP 13, MMP13.
Specifications of Rat Matrix metalloproteinase 13 ELISA kit
Product Information | |
Cat. No. | E02M0302 |
Product Name | Rat Matrix metalloproteinase 13 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MMP 13 ELISA kit uses an anti-MMP 13 antibody and an MMP 13-HRP conjugate in a competitive enzyme immunoassay method. MMP 13-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MMP 13 antibody binding site between MMP 13 from samples and MMP 13-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MMP 13. Since the number of sites is limited, as more sites are occupied by MMP 13 from the sample, fewer sites are left to bind MMP 13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 13 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Matrix metalloproteinase 13 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MMP 13 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Matrix metalloproteinase 13 ELISA kit
Citations of Rat Matrix metalloproteinase 13 ELISA kit
E02M0302 has been referenced in the below publications：
Effects of Loaded Swimming Exercise on Knee Osteoarthritis in Rats.
