Rat Matrix metalloproteinase 2 ELISA kit (E02M0304) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rat Matrix metalloproteinase 2 ELISA kit (E02M0304)

E02M0304 Rat Matrix metalloproteinase 2 ELISA kit

The Rat Matrix metalloproteinase 2 ELISA kit can be used to identify samples from the rat species. Matrix metalloproteinase 2 can also be called 72 kDa gelatinase; 72kD type IV collagenase; CLG 4; CLG 4A; Collagenase Type 4 alpha; Collagenase type IV A; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase; Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); Matrix Metalloproteinase 2; Matrix metalloproteinase II; MMP II; MONA; Neutrophil gelatinase; TBE 1, MMP2, MMP 2.

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Specifications of Rat Matrix metalloproteinase 2 ELISA kit

Product Information
Cat. No.	E02M0304
Product Name	Rat Matrix metalloproteinase 2 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/ml
Sensitivity	0.1 ng/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	1.0 ng/ml	1 vial
STANDARD C (0.5mL)	2.5 ng/ml	1 vial
STANDARD D (0.5mL)	5.0 ng/ml	1 vial
STANDARD E (0.5mL)	10 ng/ml	1 vial
STANDARD F (0.5mL)	25 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

MMP 2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MMP 2. Standards or samples are then added to the microtiter plate wells and MMP 2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MMP 2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MMP 2 are added to each well to “sandwich” the MMP 2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MMP 2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 2 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Matrix metalloproteinase 2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between MMP 2 and analogues was observed.