Rat Matrix metalloproteinase 9 ELISA kit (E02M0329) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rat Matrix metalloproteinase 9 ELISA kit (E02M0329)

E02M0329 Rat Matrix metalloproteinase 9 ELISA kit

The Rat Matrix metalloproteinase 9 ELISA kit can be used to identify samples from the rat species. Matrix metalloproteinase 9 can also be called Matrix metalloproteinase-9 precursor; 92 kDa type IV. Collagenase; 92 kDa gelatinase; Gelatinase B; GELB, MMP 9.

Products

Specifications of Rat Matrix metalloproteinase 9 ELISA kit

Product Information
Cat. No.	E02M0329
Product Name	Rat Matrix metalloproteinase 9 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/mL
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

MMP 9 ELISA kit uses an anti-MMP 9 antibody and an MMP 9-HRP conjugate in a competitive enzyme immunoassay method. MMP 9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MMP 9 antibody binding site between MMP 9 from samples and MMP 9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MMP 9. Since the number of sites is limited, as more sites are occupied by MMP 9 from the sample, fewer sites are left to bind MMP 9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 9 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Matrix metalloproteinase 9 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between MMP 9 and analogues was observed.