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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Metallothionein ELISA kit (E02M0030)

E02M0030 Rat Metallothionein ELISA kit

The Rat Metallothionein ELISA kit can be used to identify samples from the rat species. Metallothionein can also be called CES 1; MT 2;MT 2A; MT IA; MT II; MT1, MT.

Products

Specifications of Rat Metallothionein ELISA kit

Product Information

Cat. No.

E02M0030

Product Name

Rat Metallothionein ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MT. Standards or samples are then added to the microtiter plate wells and MT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MT are added to each well to “sandwich” the MT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Metallothionein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MT and analogues was observed.


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Summary of the Assay Procedure for Rat Metallothionein ELISA kit

Summary of the Assay Procedure for Rat Metallothionein ELISA kit

Citations of Rat Metallothionein ELISA kit

E02M0030 has been referenced in the below publications:

Effect of Melatonin Treatment during Pregnancy on Growth of Female Offspring in Rat.


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