E02M1085 Rat Microalbumin Elisa kit
The Rat Microalbumin Elisa kit can be used to identify samples from the rat species. Microalbumin can also be called MAU, UMA, mAlb, mALB.
Specifications of Rat Microalbumin Elisa kit
Product Information | |
Cat. No. | E02M1085 |
Product Name | Rat Microalbumin Elisa kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 mg/L |
Sensitivity | 1.0 mg/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/L | 1 vial |
STANDARD B (0.5mL) | 5.0 mg/L | 1 vial |
STANDARD C (0.5mL) | 10 mg/L | 1 vial |
STANDARD D (0.5mL) | 25 mg/L | 1 vial |
STANDARD E (0.5mL) | 50 mg/L | 1 vial |
STANDARD F (0.5mL) | 100 mg/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
mALB ELISA kit uses an anti-mALB antibody and an mALB-HRP conjugate in a competitive enzyme immunoassay method. mALB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-mALB antibody binding site between mALB from samples and mALB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of mALB. Since the number of sites is limited, as more sites are occupied by mALB from the sample, fewer sites are left to bind mALB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The mALB concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Microalbumin Elisa kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between mALB and analogues was observed. | |
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Summary of the Assay Procedure for Rat Microalbumin Elisa kit
Citations of Rat Microalbumin Elisa kit
E02M1085 has been referenced in the below publications：
电针刺激“足三里”、“曲池”穴对自发性高血压大鼠血压和肾功能影响的机制研究。
