E02M0022 Rat Microalbunminuria ELISA kit
The Rat Microalbunminuria ELISA kit can be used to identify samples from the rat species. Microalbunminuria can also be called MAU.
Specifications of Rat Microalbunminuria ELISA kit
Product Information | |
Cat. No. | E02M0022 |
Product Name | Rat Microalbunminuria ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 mg/L |
Sensitivity | 1.0 mg/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/L | 1 vial |
STANDARD B (0.5mL) | 5.0 mg/L | 1 vial |
STANDARD C (0.5mL) | 10 mg/L | 1 vial |
STANDARD D (0.5mL) | 25 mg/L | 1 vial |
STANDARD E (0.5mL) | 50 mg/L | 1 vial |
STANDARD F (0.5mL) | 100 mg/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MAU ELISA kit uses an anti-IMAU antibody and an IMAU-HRP conjugate in a competitive enzyme immunoassay method. IMAU-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IMAU antibody binding site between IMAU from samples and IMAU-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IMAU. Since the number of sites is limited, as more sites are occupied by IMAU from the sample, fewer sites are left to bind IMAU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IMAU concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Microalbunminuria ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MAU and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Microalbunminuria ELISA kit
Citations of Rat Microalbunminuria ELISA kit
E02M0022 has been referenced in the below publications：
EXPERIMENTAL STUDY OF HAIKUNSHENXI CAPSULE AND BLILING CAPSULE ON HYPERURICEMIC NEPHROPATHY.
Effect of Chinese Herbs for Stasis Removing and Collaterals Dredging upon Angiotensin-Converting Enzyme 2-Angiotensin-(1-7)Mas Axis in the Renal Cortex of Diabetic Nephropathy Rats.
EXPERIMENTAL STUDY OF HAIKUNSHENXI CAPSULE AND BLILING CAPSULE ON HYPERURICEMIC NEPHROPATHY.
EFFECT OF HAIKUNSHENXI CAPSULE ON HYPERURICEMIC NEPHROPATHY.
