Rat Mothers Against Decapentaplegic Homolog 4 ELISA kit (E02S0121)

BlueGene Biotech Rat Mothers Against Decapentaplegic Homolog 4 ELISA kit (E02S0121)

E02S0121 Rat Mothers Against Decapentaplegic Homolog 4 ELISA kit

The Rat Mothers Against Decapentaplegic Homolog 4 ELISA kit can be used to identify samples from the rat species. Mothers Against Decapentaplegic Homolog 4 can also be called DPC4, JIP, MADH4, Deletion target in pancreatic carcinoma 4, Smad4.

Products

Specifications of Rat Mothers Against Decapentaplegic Homolog 4 ELISA kit

Product Information
Cat. No.	E02S0121
Product Name	Rat Mothers Against Decapentaplegic Homolog 4 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

Smad4 ELISA kit uses an anti-Smad4 antibody and an Smad4-HRP conjugate in a competitive enzyme immunoassay method. Smad4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Smad4 antibody binding site between Smad4 from samples and Smad4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Smad4. Since the number of sites is limited, as more sites are occupied by Smad4 from the sample, fewer sites are left to bind Smad4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Smad4 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Mothers Against Decapentaplegic Homolog 4 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between Smad4 and analogues was observed.

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Summary of the Assay Procedure for Rat Mothers Against Decapentaplegic Homolog 4 ELISA kit

Citations of Rat Mothers Against Decapentaplegic Homolog 4 ELISA kit

E02S0121 has been referenced in the below publications：

The effects of JiaweiXiaokekang on the expressions of factors regulating the hepatic extracellular matrix in DN rats.

Discussion of changes of TGF-β 1，Smad-4，α-SMA and PDGF in DN rat liver before and after treatment in traditional Chinese Medicine based on the“liver and kidney share the same origin”.