E02M0347 Rat Motilin ELISA kit
The Rat Motilin ELISA kit can be used to identify samples from the rat species. Motilin can also be called Motilin, MOT, MLN, Motilin-associated peptide, Promotilin, MTL.
Specifications of Rat Motilin ELISA kit
Product Information | |
Cat. No. | E02M0347 |
Product Name | Interleukin |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pmol/L |
Sensitivity | 1.0 pmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pmol/L | 1 vial |
STANDARD B (0.5mL) | 50 pmol/L | 1 vial |
STANDARD C (0.5mL) | 100 pmol/L | 1 vial |
STANDARD D (0.5mL) | 250 pmol/L | 1 vial |
STANDARD E (0.5mL) | 500 pmol/L | 1 vial |
STANDARD F (0.5mL) | 1000 pmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MTL ELISA kit uses an anti-IMTL antibody and an IMTL-HRP conjugate in a competitive enzyme immunoassay method. IMTL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IMTL antibody binding site between IMTL from samples and IMTL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IMTL. Since the number of sites is limited, as more sites are occupied by IMTL from the sample, fewer sites are left to bind IMTL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IMTL concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Motilin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MTL and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Motilin ELISA kit
Citations of Rat Motilin ELISA kit
E02M0347 has been referenced in the below publications：
PHARMACODYNAMIC AND TOXICOLOGY STUDIES OF ZUOJIN WAN COMBINED WITH LIUJUNZI DECOCTION.
Molecular mechanisms of naloxone and opioid drugs involve in regulation of gastrointestinal motility in CNS and peripheral in a rat model of postoperative pain.
Changes of motilin concentrations in plasma and upper gastrointestinal tract in rat model of acute incisional pain.
Effects of Intrathecal Injection of Opioid Compound with Low-Dose Naloxone on Pain Behavior and Blood Motilin in a Rat Model of Incisional Pain.
