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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Mucin 2 ELISA kit (E02M0352)

  • Immunology8_copy6_copy20230526_copy20230526_copy20230526_copy20230526_copy20230526_copy20230526_copy20230526

E02M0352 Rat Mucin 2 ELISA kit

The Rat Mucin 2 ELISA kit can be used to identify samples from the rat species. Mucin 2 can also be called MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal, MUC2.

Products

Specifications of Rat Mucin 2 ELISA kit

Product Information

Cat. No.

E02M0352

Product Name

Rat Mucin 2 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MUC2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MUC2. Standards or samples are then added to the microtiter plate wells and MUC2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MUC2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MUC2 are added to each well to “sandwich” the MUC2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MUC2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC2 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Mucin 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MUC2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Mucin 2 ELISA kit

Summary of the Assay Procedure for Rat Mucin 2 ELISA kit

Citations of Rat Mucin 2 ELISA kit

E02M0352 has been referenced in the below publications:

Protective Effects and Mechanism of Erythropoietin on Necrotizing Enterocolitis in a Neonatal Rat Model.


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