E02M0034 Rat Myelin Basic Protein ELISA kit
The Rat Myelin Basic Protein ELISA kit can be used to identify samples from the rat species. Myelin Basic Protein can also be called Myelin Basic Protein, GDB, Golli MBP, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, Myelin Deficient, Myelin Membrane Encephalitogenic Protein, SHI, Shiverer, SP, MBP.
Product Information | |
Cat. No. | E02M0034 |
Product Name | Rat Myelin Basic Protein ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MBP ELISA kit uses an anti-MBP antibody and an MBP-HRP conjugate in a competitive enzyme immunoassay method. MBP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MBP antibody binding site between MBP from samples and MBP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MBP. Since the number of sites is limited, as more sites are occupied by MBP from the sample, fewer sites are left to bind MBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MBP and analogues was observed. | |
E02M0034 has been referenced in the below publications：
Mechanism of delayed encephalopathy after acute carbon monoxide intoxication in rats and intervention of dexamethasone.
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