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  • bovine serum albumin elisa 1

BlueGene Biotech Rat N Acetylgalactosamine 6 Sulfatase ELISA kit (E02G0081)

E02G0081 Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

The Rat N Acetylgalactosamine 6 Sulfatase ELISA kit can be used to identify samples from the rat species. N Acetylgalactosamine 6 Sulfatase can also be called Chondroitinase, Chondroitinsulfatase, FLJ00319, FLJ17434, FLJ42844, FLJ98217, Galactosamine (N acetyl) 6 sulfate sulfatase, Galactose 6 sulfate sulfatase, GALNAC6S, GalNAc6S sulfatase, GAS, mFLJ00319, MPS4A, N acetylgalactosamine 6 sulfatase, N acetylgalactosamine 6 sulfate sulfatase.

Products

Specifications of Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

Product Information

Cat. No.

E02G0081

Product Name

Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GAS ELISA kit uses an anti-GAS antibody and an GAS-HRP conjugate in a competitive enzyme immunoassay method. GAS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GAS antibody binding site between GAS from samples and GAS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GAS. Since the number of sites is limited, as more sites are occupied by GAS from the sample, fewer sites are left to bind GAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAS concentration in each sample is interpolated from this standard curve.


Quality Control on Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GAS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

Summary of the Assay Procedure for Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

Citations of Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

E02G0081 has been referenced in the below publications:

Effect of Liraglutide on improving the glucose metabolism by inhibiting toll-like recpter 4/ toll-like recpter 4 signaling pathway in type 2 diabetes rats.

An experimental model of rat reflux gastritis based on metabolomics.

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