Rat N Acetylgalactosamine 6 Sulfatase ELISA kit (E02G0081)

BlueGene Biotech Rat N Acetylgalactosamine 6 Sulfatase ELISA kit (E02G0081)

E02G0081 Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

The Rat N Acetylgalactosamine 6 Sulfatase ELISA kit can be used to identify samples from the rat species. N Acetylgalactosamine 6 Sulfatase can also be called Chondroitinase, Chondroitinsulfatase, FLJ00319, FLJ17434, FLJ42844, FLJ98217, Galactosamine (N acetyl) 6 sulfate sulfatase, Galactose 6 sulfate sulfatase, GALNAC6S, GalNAc6S sulfatase, GAS, mFLJ00319, MPS4A, N acetylgalactosamine 6 sulfatase, N acetylgalactosamine 6 sulfate sulfatase.

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Specifications of Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

Product Information
Cat. No.	E02G0081
Product Name	Rat N Acetylgalactosamine 6 Sulfatase ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	100-2500 pg/mL
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	100 pg/mL	1 vial
STANDARD C (0.5mL)	250 pg/mL	1 vial
STANDARD D (0.5mL)	500 pg/mL	1 vial
STANDARD E (0.5mL)	1000 pg/mL	1 vial
STANDARD F (0.5mL)	2500 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

GAS ELISA kit uses an anti-GAS antibody and an GAS-HRP conjugate in a competitive enzyme immunoassay method. GAS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GAS antibody binding site between GAS from samples and GAS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GAS. Since the number of sites is limited, as more sites are occupied by GAS from the sample, fewer sites are left to bind GAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAS concentration in each sample is interpolated from this standard curve.

Quality Control on Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between GAS and analogues was observed.

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Summary of the Assay Procedure for Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

Citations of Rat N Acetylgalactosamine 6 Sulfatase ELISA kit

E02G0081 has been referenced in the below publications：

Effect of Liraglutide on improving the glucose metabolism by inhibiting toll-like recpter 4/ toll-like recpter 4 signaling pathway in type 2 diabetes rats.

An experimental model of rat reflux gastritis based on metabolomics.