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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat N MID Osteocalcin ELISA kit (E02N0086)

E02N0086 Rat N MID Osteocalcin ELISA kit

The Rat N MID Osteocalcin ELISA kit can be used to identify samples from the rat species. N MID Osteocalcin can also be called N MID OT.

Products

Specifications of Rat N MID Osteocalcin ELISA kit

Product Information

Cat. No.

E02N0086

Product Name

Rat N MID Osteocalcin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

N MID OT ELISA kit uses an anti-N MID OT antibody and an N MID OT-HRP conjugate in a competitive enzyme immunoassay method. N MID OT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-N MID OT antibody binding site between N MID OT from samples and N MID OT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of N MID OT. Since the number of sites is limited, as more sites are occupied by N MID OT from the sample, fewer sites are left to bind N MID OT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The N MID OT concentration in each sample is interpolated from this standard curve.


Quality Control on Rat N MID Osteocalcin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between N MID OT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat N MID Osteocalcin ELISA kit

Summary of the Assay Procedure for Rat N MID Osteocalcin ELISA kit

Citations of Rat N MID Osteocalcin ELISA kit

E02N0086 has been referenced in the below publications:

Sclerostin antibody and interval treadmill training effects in a rodent model of glucocorticoid-induced osteopenia.

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