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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Neuropeptide Y ELISA kit (E02N0009)

E02N0009 Rat Neuropeptide Y ELISA kit

The Rat Neuropeptide Y ELISA kit can be used to identify samples from the rat species. Neuropeptide Y can also be called NP-Y, PYY4, CPON, Pro-neuropeptide Y, Neuropeptide tyrosine, C-flanking peptide of NPY, NPY.

Products

Specifications of Rat Neuropeptide Y ELISA kit

Product Information

Cat. No.

E02N0009

Product Name

Rat Neuropeptide Y ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NPY ELISA kit uses an anti-NPY antibody and an NPY-HRP conjugate in a competitive enzyme immunoassay method. NPY-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NPY antibody binding site between NPY from samples and NPY-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NPY. Since the number of sites is limited, as more sites are occupied by NPY from the sample, fewer sites are left to bind NPY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPY concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Neuropeptide Y ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NPY and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Neuropeptide Y ELISA kit

Summary of the Assay Procedure for Rat Neuropeptide Y ELISA kit

Citations of Rat Neuropeptide Y ELISA kit

E02N0009 has been referenced in the below publications:

Cardioprotective Effects of HuoxueAnshen Recipe against Myocardial Injuries Induced by Sleep Deprivation in Rats.

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