E02N0067 Rat Nicotinamide adenine dinucleotide phosphate ELISA kit
The Rat Nicotinamide adenine dinucleotide phosphate ELISA kit can be used to identify samples from the rat species. Nicotinamide adenine dinucleotide phosphate can also be called nicotinamide adenine dinucleotide phosphate, NADP, NADPH, TPNH, Triphosphopyridine nucleotide, reduced form, β-NADPH.
Product Information | |
Cat. No. | E02N0067 |
Product Name | Rat Nicotinamide adenine dinucleotide phosphate ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NADPH ELISA kit uses an anti-NADPH antibody and an NADPH-HRP conjugate in a competitive enzyme immunoassay method. NADPH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NADPH antibody binding site between NADPH from samples and NADPH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NADPH. Since the number of sites is limited, as more sites are occupied by NADPH from the sample, fewer sites are left to bind NADPH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NADPH concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NADPH and analogues was observed. | |
E02N0067 has been referenced in the below publications：
The Role of Resolvin D1 on acute reflux esophagitis with DHA intervention in Rats.
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