E02N0092 Rat Nitric oxide synthase ELISA kit
The Rat Nitric oxide synthase ELISA kit can be used to identify samples from the rat species. Nitric oxide synthase can also be called NOS.
Specifications of Rat Nitric oxide synthase ELISA kit
Product Information | |
Cat. No. | E02N0092 |
Product Name | Rat Nitric oxide synthase ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NOS ELISA kit uses an anti-NOS antibody and an NOS-HRP conjugate in a competitive enzyme immunoassay method. NOS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NOS antibody binding site between NOS from samples and NOS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NOS. Since the number of sites is limited, as more sites are occupied by NOS from the sample, fewer sites are left to bind NOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NOS concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Nitric oxide synthase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NOS and analogues was observed. | |
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Summary of the Assay Procedure for Rat Nitric oxide synthase ELISA kit
Citations of Rat Nitric oxide synthase ELISA kit
E02N0092 has been referenced in the below publications：
Effect of Excessive Potassium Iodide on Rat Aorta Endothelial Cells.
