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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Nitrotyrosine ELISA kit (E02N0005)

E02N0005 Rat Nitrotyrosine ELISA kit

The Rat Nitrotyrosine ELISA kit can be used to identify samples from the rat species. Nitrotyrosine can also be called 3-Nitro-L-Tyrosine, 3-Nitrotyrosine, NT.

Products

Specifications of Rat Nitrotyrosine ELISA kit

Product Information

Cat. No.

E02N0005

Product Name

Rat Nitrotyrosine ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

5.0 ng/ml

1 vial

STANDARD C (0.5mL)

10 ng/ml

1 vial

STANDARD D (0.5mL)

25 ng/ml

1 vial

STANDARD E (0.5mL)

50 ng/ml

1 vial

STANDARD F (0.5mL)

100 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for NT. Standards or samples are then added to the microtiter plate wells and NT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of NT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for NT are added to each well to “sandwich” the NT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain NT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Nitrotyrosine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Nitrotyrosine ELISA kit

Summary of the Assay Procedure for Rat Nitrotyrosine ELISA kit

Citations of Rat Nitrotyrosine ELISA kit

E02N0005 has been referenced in the below publications:

TFluoxetine is Neuroprotective in Early Brain Injury via its Anti-inflammatory and Anti-apoptotic Effects in a Rat Experimental Subarachnoid Hemorrhage Model.

Investigation into the role of Cu/Zn-SOD delivery system on its antioxidant and antiinflammatory activity in rat model of peritonitis.


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