E02N0013 Rat Noradrenaline ELISA kit
The Rat Noradrenaline ELISA kit can be used to identify samples from the rat species. Noradrenaline can also be called NA, NAd, Norepinephrine, Norepi, NADR.
Specifications of Rat Noradrenaline ELISA kit
Product Information | |
Cat. No. | E02N0013 |
Product Name | Rat Noradrenaline ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NADR ELISA kit uses an anti-NADR antibody and an NADR-HRP conjugate in a competitive enzyme immunoassay method. NADR-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NADR antibody binding site between NADR from samples and NADR-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NADR. Since the number of sites is limited, as more sites are occupied by NADR from the sample, fewer sites are left to bind NADR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NADR concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Noradrenaline ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NADR and analogues was observed. | |
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Summary of the Assay Procedure for Rat Noradrenaline ELISA kit
Citations of Rat Noradrenaline ELISA kit
E02N0013 has been referenced in the below publications：
Role of plateau hypoxia hypothermia complex damage to the vascular endothelial cells and the protective effect.
The mechanism research of neurons injury in the basolateral of amygdala induced by stress.
藜欢解郁胶囊的抗抑郁作用。
参麦黄连阿胶汤治疗绝经综合征的药效学实验。
