E02O0001 Rat Osteocalcin ELISA kit
The Rat Osteocalcin ELISA kit can be used to identify samples from the rat species. Osteocalcin can also be called Osteocalcin Propeptide; Osteocalcin; Osteocalcin precursor; BGLAP; Bone gamma carboxyglutamate protein; BGP; Bone gamma carboxyglutamate gla protein osteocalcin; Bone Gla protein; Gamma carboxyglutamic acid containing protein, OC.
Product Information | |
Cat. No. | E02O0001 |
Product Name | Rat Osteocalcin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OC ELISA kit uses an anti-OC antibody and an OC-HRP conjugate in a competitive enzyme immunoassay method. OC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OC antibody binding site between OC from samples and OC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OC. Since the number of sites is limited, as more sites are occupied by OC from the sample, fewer sites are left to bind OC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OC concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OC and analogues was observed. | |
E02O0001 has been referenced in the below publications：
The experiment study on prevention and treatment about the Zuogui Pill ovariectomized rats with osteoporosis.
Effect and Mechanism of Astragalus Total Flavonoids on Proliferation, Differentiation and Mineralization of Rat Primary Osteoblast.
Effect of Components of Jiangu Tablets in an Ovariectomized Rat Model Based on Multiple Indicator Orthogonal Experiment.
Control of MSC Differentiation by Tuning the Alkyl Chain Length of Phenylboroinc Acid Based Low-molecular-weight Gelators.
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