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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Osteocalcin ELISA kit (E02O0001)

E02O0001  Rat Osteocalcin ELISA kit

The Rat Osteocalcin ELISA kit can be used to identify samples from the rat species. Osteocalcin can also be called Osteocalcin Propeptide; Osteocalcin; Osteocalcin precursor; BGLAP; Bone gamma carboxyglutamate protein; BGP; Bone gamma carboxyglutamate gla protein osteocalcin; Bone Gla protein; Gamma carboxyglutamic acid containing protein, OC.

Products

Specifications of Rat Osteocalcin ELISA kit

Product Information

Cat. No.

E02O0001

Product Name

Rat Osteocalcin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OC ELISA kit uses an anti-OC antibody and an OC-HRP conjugate in a competitive enzyme immunoassay method. OC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OC antibody binding site between OC from samples and OC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OC. Since the number of sites is limited, as more sites are occupied by OC from the sample, fewer sites are left to bind OC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OC concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Osteocalcin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Osteocalcin ELISA kit

Summary of the Assay Procedure for Rat Osteocalcin ELISA kit

Citations of Rat Osteocalcin ELISA kit

E02O0001 has been referenced in the below publications:

The experiment study on prevention and treatment about the Zuogui Pill ovariectomized rats with osteoporosis.

Effect and Mechanism of Astragalus Total Flavonoids on Proliferation, Differentiation and Mineralization of Rat Primary Osteoblast.

Effect of Components of Jiangu Tablets in an Ovariectomized Rat Model Based on Multiple Indicator Orthogonal Experiment.

Control of MSC Differentiation by Tuning the Alkyl Chain Length of Phenylboroinc Acid Based Low-molecular-weight Gelators.



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