E02O0028 Rat Oxidized Glutathione ELISA kit
The Rat Oxidized Glutathione ELISA kit can be used to identify samples from the rat species. Oxidized Glutathione can also be called GSSG.
Specifications of Rat Oxidized Glutathione ELISA kit
Product Information | |
Cat. No. | E02O0028 |
Product Name | Rat Oxidized Glutathione ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GSSG ELISA kit uses an anti-GSSG antibody and an GSSG-HRP conjugate in a competitive enzyme immunoassay method. GSSG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GSSG antibody binding site between GSSG from samples and GSSG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GSSG. Since the number of sites is limited, as more sites are occupied by GSSG from the sample, fewer sites are left to bind GSSG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSSG concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Oxidized Glutathione ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GSSG and analogues was observed. | |
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Summary of the Assay Procedure for Rat Oxidized Glutathione ELISA kit
Citations of Rat Oxidized Glutathione ELISA kit
E02O0028 has been referenced in the below publications：
Possible mechanisms underlying fatty liver in a rat model of male hypogonadism: A protective role for testosterone.
