E02O0341 Rat Oxidized lowdensity lipoprotein ELISA kit
The Rat Oxidized lowdensity lipoprotein ELISA kit can be used to identify samples from the rat species. Oxidized lowdensity lipoprotein can also be called OL, OxLDL.
Specifications of Rat Oxidized lowdensity lipoprotein ELISA kit
Product Information | |
Cat. No. | E02O0341 |
Product Name | Rat Oxidized lowdensity lipoprotein ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ug/dl |
Sensitivity | 0.1 ug/dl |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/dl | 1 vial |
STANDARD B (0.5mL) | 2.5 ug/dl | 1 vial |
STANDARD C (0.5mL) | 5.0 ug/dl | 1 vial |
STANDARD D (0.5mL) | 10 ug/dl | 1 vial |
STANDARD E (0.5mL) | 25 ug/dl | 1 vial |
STANDARD F (0.5mL) | 50 ug/dl | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OxLDL ELISA kit uses an anti-OxLDL antibody and an OxLDL-HRP conjugate in a competitive enzyme immunoassay method. OxLDL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OxLDL antibody binding site between OxLDL from samples and OxLDL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OxLDL. Since the number of sites is limited, as more sites are occupied by OxLDL from the sample, fewer sites are left to bind OxLDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OxLDL concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Oxidized lowdensity lipoprotein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OxLDL and analogues was observed. | |
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Summary of the Assay Procedure for Rat Oxidized lowdensity lipoprotein ELISA kit
Citations of Rat Oxidized lowdensity lipoprotein ELISA kit
E02O0341 has been referenced in the below publications：
THE STUDY ON REGULATING EFFECTS AND MECHANISM OF LAMINARLA JAPONICA ON SERUM LIPID OF EXPERIMENTAL HYPERLIPENMIA.
