Rat P53 ELISA kit (E02P0041) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rat P53 ELISA kit (E02P0041)

E02P0041 Rat P53 ELISA kit

The Rat P53 ELISA kit can be used to identify samples from the rat species. P53 can also be called TP53, LFS1, TRP53, Li-Fraumeni Syndrome, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, P53.

Products

Specifications of Rat P53 ELISA kit

Product Information
Cat. No.	E02P0041
Product Name	Rat P53 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5.0 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

P53 ELISA kit uses an anti-P53 antibody and an P53-HRP conjugate in a competitive enzyme immunoassay method. P53-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-P53 antibody binding site between P53 from samples and P53-HRP conjugate, the intensity of the color is inversely proportional to the concentration of P53. Since the number of sites is limited, as more sites are occupied by P53 from the sample, fewer sites are left to bind P53-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P53 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat P53 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between P53 and analogues was observed.