E02P0088 Rat Pepsinogen C ELISA kit
The Rat Pepsinogen C ELISA kit can be used to identify samples from the rat species. Pepsinogen C can also be called PGC5; PG-C; PGC.
Specifications of Rat Pepsinogen C ELISA kit
Product Information | |
Cat. No. | E02P0088 |
Product Name | Rat Pepsinogen C ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PGC ELISA kit uses an anti-PGC antibody and an PGC-HRP conjugate in a competitive enzyme immunoassay method. PGC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PGC antibody binding site between PGC from samples and PGC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PGC. Since the number of sites is limited, as more sites are occupied by PGC from the sample, fewer sites are left to bind PGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGC concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Pepsinogen C ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PGC and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Pepsinogen C ELISA kit
Citations of Rat Pepsinogen C ELISA kit
E02P0088 has been referenced in the below publications：
Identification of Chronic Atrophic Gastritis Model Rats Differentially Expressed Proteins in Gastric Mucosa by Quantitative Proteomics and Research for Effect Targets of Chinese Medicine.
