En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Pepsinogen C ELISA kit (E02P0088)

E02P0088 Rat Pepsinogen C ELISA kit

The Rat Pepsinogen C ELISA kit can be used to identify samples from the rat species. Pepsinogen C can also be called PGC5; PG-C; PGC.

Products

Specifications of Rat Pepsinogen C ELISA kit

Product Information

Cat. No.

E02P0088

Product Name

Rat Pepsinogen C ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PGC ELISA kit uses an anti-PGC antibody and an PGC-HRP conjugate in a competitive enzyme immunoassay method. PGC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PGC antibody binding site between PGC from samples and PGC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PGC. Since the number of sites is limited, as more sites are occupied by PGC from the sample, fewer sites are left to bind PGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGC concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Pepsinogen C ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PGC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Pepsinogen C ELISA kit

Summary of the Assay Procedure for Rat Pepsinogen C ELISA kit

Citations of Rat Pepsinogen C ELISA kit

E02P0088 has been referenced in the below publications:

Identification of Chronic Atrophic Gastritis Model Rats Differentially Expressed Proteins in Gastric Mucosa by Quantitative Proteomics and Research for Effect Targets of Chinese Medicine.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.