Rat Phosphorylation Extracellular signal Regulated Kinase 1or2 ELISA kit (E02P0049)

BlueGene Biotech Rat Phosphorylation Extracellular signal Regulated Kinase 1or2 ELISA kit (E02P0049)

E02P0049 Rat Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA kit

The Rat Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA kit can be used to identify samples from the rat species. Phosphorylation Extracellular signal Regulated Kinase 1/2 can also be called MAPK1/2, P ERK1/2.

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Specifications of Rat Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA kit

Product Information
Cat. No.	E02P0049
Product Name	Rat Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5.0 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

P ERK1/2 ELISA kit uses an anti-P ERK1/2 antibody and an P ERK1/2-HRP conjugate in a competitive enzyme immunoassay method. P ERK1/2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-P ERK1/2 antibody binding site between P ERK1/2 from samples and P ERK1/2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of P ERK1/2. Since the number of sites is limited, as more sites are occupied by P ERK1/2 from the sample, fewer sites are left to bind P ERK1/2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P ERK1/2 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between P ERK1/2 and analogues was observed.

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Summary of the Assay Procedure for Rat Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA kit

Citations of Rat Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA kit

E02P0049 has been referenced in the below publications：

Dissertation Submitted to Guangzhou Sport University for Master Degree.

清肝祛湿活血方对非酒精性脂肪肝大鼠ERK1-2磷酸化的影响。