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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Plasminogen Activator Inhibitor 1 ELISA kit (E02P0028)

E02P0028 Rat Plasminogen Activator Inhibitor 1 ELISA kit

The Rat Plasminogen Activator Inhibitor 1 ELISA kit can be used to identify samples from the rat species. Plasminogen Activator Inhibitor 1 can also be called SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor.

Products

Specifications of Rat Plasminogen Activator Inhibitor 1 ELISA kit

Product Information

Cat. No.

E02P0028

Product Name

Rat Plasminogen Activator Inhibitor 1 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PAI 1 ELISA kit uses an anti-PAI 1 antibody and an PAI 1-HRP conjugate in a competitive enzyme immunoassay method. PAI 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PAI 1 antibody binding site between PAI 1 from samples and PAI 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PAI 1. Since the number of sites is limited, as more sites are occupied by PAI 1 from the sample, fewer sites are left to bind PAI 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAI 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Plasminogen Activator Inhibitor 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PAI 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Plasminogen Activator Inhibitor 1 ELISA kit

Summary of the Assay Procedure for Rat Plasminogen Activator Inhibitor 1 ELISA kit

Citations of Rat Plasminogen Activator Inhibitor 1 ELISA kit

E02P0028 has been referenced in the below publications:

Effect of different compatibility of huayu-tongluo Chinese herbs on the kidney of diabetic nephropathy rats the expression of integrin alpha 3 beta 1 and the related proteins in wnt/beta-catenin pathway.

Study on Hemostatic Effect and Mechanism of Polysaccharides from Bletilla striata in Blood Heat and Hemorrhage Model Rats.

Influence of Endothelial Function and Blood Rheology in Diabetic Rats by Paeonol and Tanshinol.


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