E02P0127 Rat Plasminogen ELISA kit
The Rat Plasminogen ELISA kit can be used to identify samples from the rat species. Plasminogen can also be called PL, Plasmin, Activation peptide, Angiostatin, Plg.
Specifications of Rat Plasminogen ELISA kit
Product Information | |
Cat. No. | E02P0127 |
Product Name | Rat Plasminogen ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Plg ELISA kit uses an anti-IPlg antibody and an IPlg-HRP conjugate in a competitive enzyme immunoassay method. IPlg-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IPlg antibody binding site between IPlg from samples and IPlg-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IPlg. Since the number of sites is limited, as more sites are occupied by IPlg from the sample, fewer sites are left to bind IPlg-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IPlg concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Plasminogen ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Plg and analogues was observed. | |
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Summary of the Assay Procedure for Rat Plasminogen ELISA kit
Citations of Rat Plasminogen ELISA kit
E02P0127 has been referenced in the below publications：
Study on Mechanism of Yiqi Huoxue Compound Anti-arterial Thrombosis and Its Action.
