E02P0578 Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit
The Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit can be used to identify samples from the rat species. Procollagen Type Ⅰ N Terminal Propeptide can also be called P1NP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide.
Product Information | |
Cat. No. | E02P0578 |
Product Name | Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PINP ELISA kit uses an anti-PINP antibody and an PINP-HRP conjugate in a competitive enzyme immunoassay method. PINP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PINP antibody binding site between PINP from samples and PINP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PINP. Since the number of sites is limited, as more sites are occupied by PINP from the sample, fewer sites are left to bind PINP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PINP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PINP and analogues was observed. | |
E02P0578 has been referenced in the below publications：
The effects of estrogen and PTH on spinal Wnt/β-catenin pathway and degeneration in ovariectomized rats.
Estrogen alone or in combination with parathyroid hormone can decrease vertebral MEF2 and sclerostin expression and increase vertebral bone mass in ovariectomized rats.
The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats.
Involvement of periostin-sclerostin-Wnt/β-catenin signaling pathway in the prevention of neurectomy-induced bone loss by naringin.
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