E02P0200 Rat Progesterone ELISA kit
The Rat Progesterone ELISA kit can be used to identify samples from the rat species. Progesterone can also be called P4, Pregn-4-Ene-3,20-Dione, PROG.
Specifications of Rat Progesterone ELISA kit
Product Information | |
Cat. No. | E02P0200 |
Product Name | Rat Progesterone ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PROG ELISA kit uses an anti-PROG antibody and an PROG-HRP conjugate in a competitive enzyme immunoassay method. PROG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PROG antibody binding site between PROG from samples and PROG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PROG. Since the number of sites is limited, as more sites are occupied by PROG from the sample, fewer sites are left to bind PROG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PROG concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Progesterone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PROG and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Progesterone ELISA kit
Citations of Rat Progesterone ELISA kit
E02P0200 has been referenced in the below publications：
益肾健脾通利活血方治疗痰湿型闭经理论与实验研究。
THE EFFECTS OF TESTOSTERONE ON FUNCTION AND STRUCTURE OF KIDNEY AND ARTERY IN SPONTANEOUSLY HYPERTENSIVE RATS.
Effects of high exposure of endogenous reproductive hormones on perimenopausal and postmenopausal blood pressure in rats.
Correlation between Expression of GABAA Extrasynaptic Receptors in Hippocampus Dentate Gyrus,Amygdala and Alterations of Estrogen and Progesterone during Rat Estrus Cycle.
Establishment of in-vitro three dimensional rat follicle culture system and validation of the applicability as an in vitro female reproductive toxicity testing system.
