E02P0027 Rat Prostaglandin ELISA
The Rat Prostaglandin ELISA can be used to identify samples from the rat species. Prostaglandin can also be called PRD, Xaa-Pro Dipeptidase, Prolidase, Imidodipeptidase, Proline dipeptidase, PEPD.
Specifications of Rat Prostaglandin ELISA
Product Information | |
Cat. No. | E02P0027 |
Product Name | Rat Prostaglandin ELISA |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PEPD ELISA kit uses an anti-PEPD antibody and an PEPD-HRP conjugate in a competitive enzyme immunoassay method. PEPD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PEPD antibody binding site between PEPD from samples and PEPD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PEPD. Since the number of sites is limited, as more sites are occupied by PEPD from the sample, fewer sites are left to bind PEPD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PEPD concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Prostaglandin ELISA
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PEPD and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Prostaglandin ELISA
Citations of Rat Prostaglandin ELISA
E02P0027 has been referenced in the below publications：
Study on the treatment of chronic superficial gastritis treated with Jin Qi capsule and its main effect
