E02S0159 Rat Pulmonary Surfactant Associated Protein A ELISA kit
The Rat Pulmonary Surfactant Associated Protein A ELISA kit can be used to identify samples from the rat species. Pulmonary Surfactant Associated Protein A can also be called PRL, SFTPA, PSAP, PSPA, SFTP, SPA, COLEC4, PSP-A, SFTP1, SFTPA1B, SP-A1, Collectin-4, Alveolar proteinosis protein, 35 kDa pulmonary surfactant-associated protein, Surfactant Associated Protein A.
Product Information | |
Cat. No. | E02S0159 |
Product Name | Rat Pulmonary Surfactant Associated Protein A ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SPA ELISA kit uses an anti-ISPA antibody and an ISPA-HRP conjugate in a competitive enzyme immunoassay method. ISPA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ISPA antibody binding site between ISPA from samples and ISPA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ISPA. Since the number of sites is limited, as more sites are occupied by ISPA from the sample, fewer sites are left to bind ISPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ISPA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SPA and analogues was observed. | |
E02S0159 has been referenced in the below publications：
Effect of passive smoking on the expression of surfactant protein A and D in rats.
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