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BlueGene Biotech Rat Pulmonary Surfactant Associated Protein A ELISA kit (E02S0159)

E02S0159 Rat Pulmonary Surfactant Associated Protein A ELISA kit

The Rat Pulmonary Surfactant Associated Protein A ELISA kit can be used to identify samples from the rat species. Pulmonary Surfactant Associated Protein A can also be called PRL, SFTPA, PSAP, PSPA, SFTP, SPA, COLEC4, PSP-A, SFTP1, SFTPA1B, SP-A1, Collectin-4, Alveolar proteinosis protein, 35 kDa pulmonary surfactant-associated protein, Surfactant Associated Protein A.

Products

Specifications of Rat Pulmonary Surfactant Associated Protein A ELISA kit

Product Information

Cat. No.

E02S0159

Product Name

Rat Pulmonary Surfactant Associated Protein A ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SPA ELISA kit uses an anti-ISPA antibody and an ISPA-HRP conjugate in a competitive enzyme immunoassay method. ISPA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ISPA antibody binding site between ISPA from samples and ISPA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ISPA. Since the number of sites is limited, as more sites are occupied by ISPA from the sample, fewer sites are left to bind ISPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ISPA concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Pulmonary Surfactant Associated Protein A ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SPA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Pulmonary Surfactant Associated Protein A ELISA kit

Summary of the Assay Procedure for Rat Pulmonary Surfactant Associated Protein A ELISA kit

Citations of Rat Pulmonary Surfactant Associated Protein A ELISA kit

E02S0159 has been referenced in the below publications:

Effect of passive smoking on the expression of surfactant protein A and D in rats.


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