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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Resistin ELISA kit (E02R0351)

E02R0351 Rat Resistin ELISA kit

The Rat Resistin ELISA kit can be used to identify samples from the rat species. Resistin can also be called Adipose tissue specific secretory factor, ADSF, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1, Cysteine rich secreted protein A12 alpha like 2, Cysteine rich secreted protein FIZZ3, FIZZ 3, Found in inflammatory zone 3, HXCP 1, HXCP1, Resistin delta2, RETN 1, RETN, RETN1, RSTN, XCP 1, XCP1.

Products

Specifications of Rat Resistin ELISA kit

Product Information

Cat. No.

E02R0351

Product Name

Rat Resistin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Resistin ELISA kit uses an anti-Resistin antibody and an Resistin-HRP conjugate in a competitive enzyme immunoassay method. Resistin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Resistin antibody binding site between Resistin from samples and Resistin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Resistin. Since the number of sites is limited, as more sites are occupied by Resistin from the sample, fewer sites are left to bind Resistin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Resistin concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Resistin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Resistin and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Resistin ELISA kit

Summary of the Assay Procedure for Rat Resistin ELISA kit

Citations of Rat Resistin ELISA kit

E02R0351 has been referenced in the below publications:

Qingrejiedu party of type 2 diabetic rats serum inflammation factors leptin IL resistance element-1 intervention study.

The efficacy of quercetin on the expression of resistin and insulin resistance in rats with non-alcoholic fatty liver disease..

Impact of Tangzhiping on Lipid Metabolism and Adipokine in the Rats of Insulin Resistance.


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