E02R0474 Rat Resolvin D1 ELISA kit
The Rat Resolvin D1 ELISA kit ELISA kit can be used to identify samples from the rat species. Resolvin D1 can also be called 17(S)-Resolvin D1, RvD1.
Specifications of Rat Resolvin D1 ELISA kit
Product Information | |
Cat. No. | E02R0474 |
Product Name | Rat Resolvin D1 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
RvD1 ELISA kit uses an anti-RvD1 antibody and an RvD1-HRP conjugate in a competitive enzyme immunoassay method. RvD1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-RvD1 antibody binding site between RvD1 from samples and RvD1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of RvD1. Since the number of sites is limited, as more sites are occupied by RvD1 from the sample, fewer sites are left to bind RvD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RvD1 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Resolvin D1 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between RvD1 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Resolvin D1 ELISA kit
Citations of Rat Resolvin D1 ELISA kit
E02R0474 has been referenced in the below publications：
The Role of Resolvin D1 on acute reflux esophagitis with DHA intervention in Rats.
