E02R0001 Rat Retinol Binding Protein 4 ELISA kit
The Rat Retinol Binding Protein 4 ELISA kit can be used to identify samples from the rat species. Retinol Binding Protein 4 can also be called RBP4, MCOPCB10, RDCCAS, retinol binding protein 4.
Specifications of Rat Retinol Binding Protein 4 ELISA kit
Product Information | |
Cat. No. | E02R0001 |
Product Name | Rat Retinol Binding Protein 4 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD E (0.5mL) | 10 ug/mL | 1 vial |
STANDARD F (0.5mL) | 25 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
RBP4 ELISA kit uses an anti-RBP4 antibody and an RBP4-HRP conjugate in a competitive enzyme immunoassay method. RBP4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-RBP4 antibody binding site between RBP4 from samples and RBP4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of RBP4. Since the number of sites is limited, as more sites are occupied by RBP4 from the sample, fewer sites are left to bind RBP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RBP4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Retinol Binding Protein 4 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between RBP4 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Retinol Binding Protein 4 ELISA kit
Citations of Rat Retinol Binding Protein 4 ELISA kit
E02R0001 has been referenced in the below publications：
The correlation of insulin-resistance and RBP4 in rat with type 2 diabetes.
Choroid plexus aquaporin 1 and intracranial pressure are increased in obese rats: towards an idiopathic intracranial hypertension model.
Increased retinol-free RBP4 contributes to insulin resistance in gestational diabetes mellitus.
