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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Ribosomal Protein S19 ELISA (E02R0076)

  • Epigenetics and Nuclear Signaling

E02R0076 Rat Ribosomal Protein S19 ELISA

The Rat Ribosomal Protein S19 ELISA can be used to identify samples from the rat species. Ribosomal Protein S19 can also be called DBA, DBA1, S19, Ribosomal protein S19, eS19, LOH19CR1, RPS19.

Products

Specifications of Rat Ribosomal Protein S19 ELISA

Product Information

Cat. No.

E02R0076

Product Name

Rat Ribosomal Protein S19 ELISA

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

RPS19 ELISA kit uses an anti-RPS19 antibody and an RPS19-HRP conjugate in a competitive enzyme immunoassay method. RPS19-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-RPS19 antibody binding site between RPS19 from samples and RPS19-HRP conjugate, the intensity of the color is inversely proportional to the concentration of RPS19. Since the number of sites is limited, as more sites are occupied by RPS19 from the sample, fewer sites are left to bind RPS19-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPS19 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Ribosomal Protein S19 ELISA

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between RPS19 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Ribosomal Protein S19 ELISA

Summary of the Assay Procedure for Rat Ribosomal Protein S19 ELISA

Citations of Rat Ribosomal Protein S19 ELISA

E02R0076 has been referenced in the below publications:

Treatment with Cardiotonic Pills ® after IschemiaReperfusion Ameliorates Myocardial Fibrosis in Rats.


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