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BlueGene Biotech Rat Sex Hormone Binding Globulin ELISA kit (E02S0026)

E02S0026 Rat Sex Hormone Binding Globulin ELISA kit

The Rat Sex Hormone Binding Globulin ELISA kit can be used to identify samples from the rat species. Sex Hormone Binding Globulin can also be called SHBG, ABP, SBP, TEBG, sex hormone binding globulin, Sex hormone-binding globulin.

Products

Specifications of Rat Sex Hormone Binding Globulin ELISA kit

Product Information

Cat. No.

E02S0026

Product Name

Rat Sex Hormone Binding Globulin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

10-250 nmol/L

Sensitivity

1.0 nmol/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 nmol/L

1 vial

STANDARD B (0.5mL)

10 nmol/L

1 vial

STANDARD C (0.5mL)

25 nmol/L

1 vial

STANDARD D (0.5mL)

50 nmol/L

1 vial

STANDARD E (0.5mL)

100 nmol/L

1 vial

STANDARD F (0.5mL)

250 nmol/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SHBG ELISA kit uses an anti-ISHBG antibody and an ISHBG-HRP conjugate in a competitive enzyme immunoassay method. ISHBG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ISHBG antibody binding site between ISHBG from samples and ISHBG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ISHBG. Since the number of sites is limited, as more sites are occupied by ISHBG from the sample, fewer sites are left to bind ISHBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ISHBG concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Sex Hormone Binding Globulin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SHBG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Sex Hormone Binding Globulin ELISA kit

Summary of the Assay Procedure for Rat Sex Hormone Binding Globulin ELISA kit

Citations of Rat Sex Hormone Binding Globulin ELISA kit

E02S0026 has been referenced in the below publications:

The study of rat polycystic ovarian syndrome(PCOS) model induced by sodium prasterone surfate.


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