E02S0026 Rat Sex Hormone Binding Globulin ELISA kit
The Rat Sex Hormone Binding Globulin ELISA kit can be used to identify samples from the rat species. Sex Hormone Binding Globulin can also be called SHBG, ABP, SBP, TEBG, sex hormone binding globulin, Sex hormone-binding globulin.
Product Information | |
Cat. No. | E02S0026 |
Product Name | Rat Sex Hormone Binding Globulin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 nmol/L |
Sensitivity | 1.0 nmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 nmol/L | 1 vial |
STANDARD B (0.5mL) | 10 nmol/L | 1 vial |
STANDARD C (0.5mL) | 25 nmol/L | 1 vial |
STANDARD D (0.5mL) | 50 nmol/L | 1 vial |
STANDARD E (0.5mL) | 100 nmol/L | 1 vial |
STANDARD F (0.5mL) | 250 nmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SHBG ELISA kit uses an anti-ISHBG antibody and an ISHBG-HRP conjugate in a competitive enzyme immunoassay method. ISHBG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ISHBG antibody binding site between ISHBG from samples and ISHBG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ISHBG. Since the number of sites is limited, as more sites are occupied by ISHBG from the sample, fewer sites are left to bind ISHBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ISHBG concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SHBG and analogues was observed. | |
E02S0026 has been referenced in the below publications：
The study of rat polycystic ovarian syndrome(PCOS) model induced by sodium prasterone surfate.
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