E02S0457 Rat soluble Stromelysin 2 ELISA kit
The Rat soluble Stromelysin 2 ELISA kit can be used to identify samples from the rat species. soluble Stromelysin 2 can also be called matrix metalloproteinase 10, transin 2, proteoglycanase 2, sST2.
Specifications of Rat soluble Stromelysin 2 ELISA kit
Product Information | |
Cat. No. | E02S0457 |
Product Name | Rat soluble Stromelysin 2 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
sST2 ELISA kit uses an anti-sST2 antibody and an sST2-HRP conjugate in a competitive enzyme immunoassay method. sST2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sST2 antibody binding site between sST2 from samples and sST2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sST2. Since the number of sites is limited, as more sites are occupied by sST2 from the sample, fewer sites are left to bind sST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sST2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat soluble Stromelysin 2 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sST2 and analogues was observed. | |
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Summary of the Assay Procedure for Rat soluble Stromelysin 2 ELISA kit
Citations of Rat soluble Stromelysin 2 ELISA kit
E02S0457 has been referenced in the below publications：
Clinical Research about B-brain Natriuretic Peptide,Soluble ST2 and Interleukin 33 in Patients with Heart Failure.
