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BlueGene Biotech Rat Stromelysin-2 ELISA (E02S0299)

E02S0299 Rat Stromelysin-2 ELISA

The Rat Stromelysin-2 ELISA can be used to identify samples from the rat species. Stromelysin-2 can also be called MMP-10, MMP10, SL-2, STMY2, Stromelysin-2, Transin-2.

Products

Specifications of Rat Stromelysin-2 ELISA

Product Information

Cat. No.

E02S0299

Product Name

Rat Stromelysin-2 ELISA

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ST2 ELISA kit uses an anti-ST2 antibody and an ST2-HRP conjugate in a competitive enzyme immunoassay method. ST2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ST2 antibody binding site between ST2 from samples and ST2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ST2. Since the number of sites is limited, as more sites are occupied by ST2 from the sample, fewer sites are left to bind ST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ST2 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Stromelysin-2 ELISA

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ST2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Stromelysin-2 ELISA

Summary of the Assay Procedure for Rat Stromelysin-2 ELISA

Citations of Rat Stromelysin-2 ELISA

E02S0299 has been referenced in the below publications:

Effects of exercise traning on ventricular remodeling following myocardial infarction in rats.

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