E02S0012 Rat Superoxide Dismutase ELISA kit
The Rat Superoxide Dismutase ELISA kit can be used to identify samples from the rat species. Superoxide Dismutase can also be called SOD1, Cu-Zn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble, ALS, ALS1, amyotrophic lateral sclerosis 1, adult, Cu, Zn superoxide dismutase, hSod1, indophenoloxidase A, IPOA, SOD, Superoxide dismutase 1, superoxide dismutase 1, soluble, superoxide dismutase, cystolic.
Product Information | |
Cat. No. | E02S0012 |
Product Name | Rat Superoxide Dismutase ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD E (0.5mL) | 10 ug/mL | 1 vial |
STANDARD F (0.5mL) | 25 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SOD ELISA kit uses an anti-SOD antibody and an SOD-HRP conjugate in a competitive enzyme immunoassay method. SOD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SOD antibody binding site between SOD from samples and SOD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SOD. Since the number of sites is limited, as more sites are occupied by SOD from the sample, fewer sites are left to bind SOD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SOD and analogues was observed. | |
E02S0012 has been referenced in the below publications：
Control study of the effects of curcumin and pioglitazone in nonalcoholic steatohepatitis in rats.
The protective effect of p38MAPK inhibitor on the paraquat-induced acute lung injury in rats.
Effects of rapid entrance into plateau area on the vascular endothelial function and the relationship to acute mountain sickness.
Beneficial Effects of Lactobacillus plantarum Strains on Non-Alcoholic Fatty Liver Disease in High Fat/High Fructose Diet-Fed Rats.
Gastroprotective Effects of Fermented Lotus Root against Ethanol/HCl-Induced Gastric Mucosal Acute Toxicity in Rats.
Liver injury attenuation by curcumin in a rat NASH model: an Nrf2 activation-mediated effect?.
Comparison of Pancreatic Microcirculation Profiles in Spontaneously Hypertensive Rats and Wistar-Kyoto Rats by Laser Doppler and Wavelet Transform Analysis.
Effects of Schiff base aromatic amino acid derivatives on antioxidant and immune system disturbances in a rat model of aflatoxin B1 induced experimental mycotoxicosis.
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