E02S0272 Rat Surfactant P ELISA kit
The Rat Surfactant P ELISA kit can be used to identify samples from the rat species. Surfactant P can also be called SP.
Specifications of Rat Surfactant P ELISA kit
Product Information | |
Cat. No. | E02S0272 |
Product Name | Rat Surfactant P ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SP ELISA kit uses an anti-SP antibody and an SP-HRP conjugate in a competitive enzyme immunoassay method. SP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SP antibody binding site between SP from samples and SP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SP. Since the number of sites is limited, as more sites are occupied by SP from the sample, fewer sites are left to bind SP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SP concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Surfactant P ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SP and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Surfactant P ELISA kit
Citations of Rat Surfactant P ELISA kit
E02S0272 has been referenced in the below publications：
Effects of ischemic preconditioning on expression of VEGF, GLUT1 and BAI1 in focal cerebral infarction rats.
Effects and mechanism ofelectro-acupuncture on constipation and diarrhea type of irritable bowel syndrome in rats.
Effects of KuiKeLing and Its Modified Formula on Bowel Movement and Gastrointestinal Peptide Hormones in Colitis Model Rats.
The Liver Injury Mechamisms In Rats With Sever Acute Pancreatitis And Protective Effects Of N-acetylcysteine Homocysteine To Liver.
Downregulated microRNA-92a-3p inhibits apoptosis and promotes proliferation of pancreatic acinar cells in acute pancreatitis by enhancing KLF2 expression.
