E02S0301 Rat Syndecan 1 ELISA kit
The Rat Syndecan 1 ELISA kit can be used to identify samples from the rat species. Syndecan 1 can also be called SDC1, CD138, SDC, SYND1, syndecan, syndecan 1.
Specifications of Rat Syndecan 1 ELISA kit
Product Information | |
Cat. No. | E02S0301 |
Product Name | Rat Syndecan 1 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SDC1/CD138 ELISA kit uses an anti-SDC1/CD138 antibody and an SDC1/CD138-HRP conjugate in a competitive enzyme immunoassay method. SDC1/CD138-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SDC1/CD138 antibody binding site between SDC1/CD138 from samples and SDC1/CD138-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SDC1/CD138. Since the number of sites is limited, as more sites are occupied by SDC1/CD138 from the sample, fewer sites are left to bind SDC1/CD138-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDC1/CD138 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Syndecan 1 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SDC1/CD138 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Syndecan 1 ELISA kit
Citations of Rat Syndecan 1 ELISA kit
E02S0301 has been referenced in the below publications：
Impact of b-Adrenoceptor Blockade on Systemic Inflammation and Coagulation Disturbances in Rats with Acute Traumatic Coagulopathy.
Both UFH and NAH alleviate shedding of endothelial glycocalyx and coagulopathy in LPS‑induced sepsis.
