E02T0912 Rat taurochenodeoxycholic acid ELISA kit
The Rat taurochenodeoxycholic acid ELISA kit can be used to identify samples from the rat species. taurochenodeoxycholic acid can also be called 12-Deoxycholyltaurine, 12-Desoxycholyltaurine, Chenodeoxycholyltaurine, Chenyltaurine, TCDCA.
Product Information | |
Cat. No. | E02T0912 |
Product Name | Rat taurochenodeoxycholic acid ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 10 ng/ml | 1 vial |
STANDARD E (0.5mL) | 25 ng/ml | 1 vial |
STANDARD F (0.5mL) | 50 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TCDCA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TCDCA. Standards or samples are then added to the microtiter plate wells and TCDCA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TCDCA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TCDCA are added to each well to “sandwich” the TCDCA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TCDCA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TCDCA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TCDCA and analogues was observed. | |
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