E02T0007 Rat Testosterone ELISA kit
The Rat Testosterone ELISA kitcan be used to identify samples from the rat species. Testosterone can also be called Testesteron, Testeronun, TESTO.
Specifications of Rat Testosterone ELISA kit
Product Information | |
Cat. No. | E02T0007 |
Product Name | Rat Testosterone ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TESTO ELISA kit uses an anti-TESTO antibody and an TESTO-HRP conjugate in a competitive enzyme immunoassay method. TESTO-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TESTO antibody binding site between TESTO from samples and TESTO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TESTO. Since the number of sites is limited, as more sites are occupied by TESTO from the sample, fewer sites are left to bind TESTO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TESTO concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Testosterone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TESTO and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Testosterone ELISA kit
Citations of Rat Testosterone ELISA kit
E02T0007 has been referenced in the below publications：
The study of rat polycystic ovarian syndrome(PCOS) model induced by sodium prasterone surfate．
Influences of Maca and Tongkat Ali on The Serum Testosterone Level of Swimming Training Rats.
益肾健脾通利活血方治疗痰湿型闭经理论与实验研究。
THE EFFECTS OF TESTOSTERONE ON FUNCTION AND STRUCTURE OF KIDNEY AND ARTERY IN SPONTANEOUSLY HYPERTENSIVE RATS.
低强度耐力运动对被动吸烟大鼠睾丸相关酶活性的影响。
Testosterone improved the aortic vascular structure and function of rats in D-galactose-induced rat model of aging.
The Influence of Gibberellic Acid Exposure on the Pubertal Development of SD Rats.
东革阿里对游泳训练大鼠血睾酮水平及运动能力的影响。
Physcion, a novel inhibitor of 5α-reductase that promotes hair growth in vitro and in vivo.
Testosterone regulates myosin II isoforms expression and functional activity in the rat prostate.
Amelioration of Nandrolone Decanoate-Induced Testicular and Sperm Toxi- city in Rats by Taurine: Effects on Steroidogenesis, Redox and Inflammatory Cascades, and Intrinsic Apoptotic Pathway.
Alteration of the Expression and Functional Activities of Myosin II Isoforms in Enlarged Hyperplastic Prostates.
