E02T0336 Rat Thioredoxin ELISA kit
The Rat Thioredoxin ELISA kit can be used to identify samples from the rat species. Carcinoembryonic can also be called TXN, ADF, SASP, TRDX, TRX1, ATL-derived factor, Surface-associated sulphydryl protein, Trx.
Specifications of Rat Thioredoxin ELISA kit
Product Information | |
Cat. No. | E02T0336 |
Product Name | Rat Thioredoxin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 100 pg/ml | 1 vial |
STANDARD C (0.5mL) | 250 pg/ml | 1 vial |
STANDARD D (0.5mL) | 500 pg/ml | 1 vial |
STANDARD E (0.5mL) | 1000 pg/ml | 1 vial |
STANDARD F (0.5mL) | 2500 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Trx ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Trx. Standards or samples are then added to the microtiter plate wells and Trx if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Trx present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Trx are added to each well to “sandwich” the Trx immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Trx and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Trx concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Thioredoxin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Trx and analogues was observed. | |
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Summary of the Assay Procedure for Rat Thioredoxin ELISA kit
Citations of Rat Thioredoxin ELISA kit
E02T0336 has been referenced in the below publications：
The role of oxidative stress caused by different feeding manners in the formation of emphysema in rats.
