E02T0025 Rat Thromboxanes B2 ELISA kit
The Rat Thromboxanes B2 ELISA kit can be used to identify samples from the rat species. Thromboxanes B2 can also be called TXB2, hromboxane-b, Thromboxane B2, TXB 2.
Specifications of Rat Thromboxanes B2 ELISA kit
Product Information | |
Cat. No. | E02T0025 |
Product Name | Rat Thromboxanes B2 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TXB2 ELISA kit uses an anti-TXB2 antibody and an TXB2-HRP conjugate in a competitive enzyme immunoassay method. TXB2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TXB2 antibody binding site between TXB2 from samples and TXB2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TXB2. Since the number of sites is limited, as more sites are occupied by TXB2 from the sample, fewer sites are left to bind TXB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TXB2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Thromboxanes B2 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TXB2 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Thromboxanes B2 ELISA kit
Citations of Rat Thromboxanes B2 ELISA kit
E02T0025 has been referenced in the below publications：
Influence of angelica essential oil aromatherapy on rats model of dysmenorrhea.
Study on Mechanism of Yiqi Huoxue Compound Anti-arterial Thrombosis and Its Action.
Experimental animal model to study iron overload and iron chelation and review of other such models.
