En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Thyroglobulin Antibody ELISA kit (E02T0767)

E02T0767 Rat Thyroglobulin Antibody ELISA kit

The Rat Thyroglobulin Antibody ELISA kit  can be used to identify samples from the rat species. Thyroglobulin Antibody can also be called TG Antibody, Thyroglobulin Antibody, AITD3 Antibody, cog Antibody, TDH3 Antibody, TGN Antibody, AITD3TGN Antibody, TDH3 Antibody, TGAb.

Products

Specifications of Rat Thyroglobulin Antibody ELISA kit

Product Information

Cat. No.

E02T0767

Product Name

Rat Thyroglobulin Antibody ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TGAb ELISA kit uses an anti-TGAb antibody and an TGAb-HRP conjugate in a competitive enzyme immunoassay method. TGAb-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TGAb antibody binding site between TGAb from samples and TGAb-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TGAb. Since the number of sites is limited, as more sites are occupied by TGAb from the sample, fewer sites are left to bind TGAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGAb concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Thyroglobulin Antibody ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TGAb and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Thyroglobulin Antibody Elisa Kit

Summary of the Assay Procedure for Rat Thyroglobulin Antibody Elisa Kit

Citations of Rat Thyroglobulin Antibody ELISA kit

E02T0767 has been referenced in the below publications:

Exposure to DBP and high iodine aggravates autoimmune thyroid disease through increasing the levels of IL-17 and thyroid-binding globulin in wistar rats.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.