E02T0022 Rat Thyroid stimulating hormone ELISA kit
The Rat Thyroid stimulating hormone ELISA kit can be used to identify samples from the rat species. Thyroid stimulating hormone can also be called Thyrotropin, TSH.
Specifications of Rat Thyroid stimulating hormone ELISA kit
Product Information | |
Cat. No. | E02T0022 |
Product Name | Rat Thyroid stimulating hormone ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TSH ELISA kit uses an anti-TSH antibody and an TSH-HRP conjugate in a competitive enzyme immunoassay method. TSH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TSH antibody binding site between TSH from samples and TSH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TSH. Since the number of sites is limited, as more sites are occupied by TSH from the sample, fewer sites are left to bind TSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TSH concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Thyroid stimulating hormone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TSH and analogues was observed. | |
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Summary of the Assay Procedure for Rat Thyroid stimulating hormone ELISA kit
Citations of Rat Thyroid stimulating hormone ELISA kit
E02T0022 has been referenced in the below publications：
The Influence of Gibberellic Acid Exposure on the Pubertal Development of SD Rat.
