E02T0504 Rat Total bile acide ELISA kit
The Rat Total bile acide ELISA kit can be used to identify samples from the rat species. Total bile acide can also be called TBAM.
Specifications of Rat Total bile acide ELISA kit
Product Information | |
Cat. No. | E02T0504 |
Product Name | Rat Total bile acide ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 µmol/L |
Sensitivity | 0.1 µmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 µmol/L | 1 vial |
STANDARD B (0.5mL) | 0.5 µmol/L | 1 vial |
STANDARD C (0.5mL) | 1.0 µmol/L | 1 vial |
STANDARD D (0.5mL) | 2.5 µmol/L | 1 vial |
STANDARD E (0.5mL) | 5.0 µmol/L | 1 vial |
STANDARD F (0.5mL) | 10 µmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TBAM ELISA kit uses an anti-ITBAM antibody and an ITBAM-HRP conjugate in a competitive enzyme immunoassay method. ITBAM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ITBAM antibody binding site between ITBAM from samples and ITBAM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ITBAM. Since the number of sites is limited, as more sites are occupied by ITBAM from the sample, fewer sites are left to bind ITBAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITBAM concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Total bile acide ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TBAM and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Total bile acide ELISA kit
Citations of Rat Total bile acide ELISA kit
E02T0504 has been referenced in the below publications：
Oral administration of oleanolic acid, isolated from Swertia mussotii Franch, attenuates liver injury,inflammation, and cholestasis in bile duct-ligated rats.
Swertianlarin, isolated from Swertia mussotii Franch, increases detoxification enzymes and efflux transporters expression in rats.
