E02T0020 Rat Total Cholesterol ELISA kit
The Rat Total Cholesterol ELISA kit can be used to identify samples from the rat species. Total Cholesterol can also be called Cholesterin, Cholesteryl alcohol, TC.
Specifications of Rat Total Cholesterol ELISA kit
Product Information | |
Cat. No. | E02T0020 |
Product Name | Rat Total Cholesterol ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 mmol/L |
Sensitivity | 0.1 mmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mmol/L | 1 vial |
STANDARD B (0.5mL) | 0.5 mmol/L | 1 vial |
STANDARD C (0.5mL) | 1.0 mmol/L | 1 vial |
STANDARD D (0.5mL) | 2.5 mmol/L | 1 vial |
STANDARD E (0.5mL) | 5.0 mmol/L | 1 vial |
STANDARD F (0.5mL) | 10 mmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TC ELISA kit uses an anti-TC antibody and an TC-HRP conjugate in a competitive enzyme immunoassay method. TC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TC antibody binding site between TC from samples and TC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TC. Since the number of sites is limited, as more sites are occupied by TC from the sample, fewer sites are left to bind TC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TC concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Total Cholesterol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TC and analogues was observed. | |
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Summary of the Assay Procedure for Rat Total Cholesterol ELISA kit
Citations of Rat Total Cholesterol ELISA kit
E02T0020 has been referenced in the below publications：
Study on Antioxidation and Reducing Blood Lipid of Chilli Seed Oil.
