E02H0031 Rat Total Homocystein ELISA kit
The Rat Total Homocystein ELISA kit can be used to identify samples from the rat species. Total Homocystein can also be called Homocysteine, Hcy, HCY.
Specifications of Rat Total Homocystein ELISA kit
Product Information | |
Cat. No. | E02H0031 |
Product Name | Rat Total Homocystein ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 μmol/L |
Sensitivity | 1.0 umol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 umol/L | 1 vial |
STANDARD B (0.5mL) | 50 umol/L | 1 vial |
STANDARD C (0.5mL) | 100 umol/L | 1 vial |
STANDARD D (0.5mL) | 250 umol/L | 1 vial |
STANDARD E (0.5mL) | 500 umol/L | 1 vial |
STANDARD F (0.5mL) | 1000 umol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HCY ELISA kit uses an anti-IHCY antibody and an IHCY-HRP conjugate in a competitive enzyme immunoassay method. IHCY-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IHCY antibody binding site between IHCY from samples and IHCY-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IHCY. Since the number of sites is limited, as more sites are occupied by IHCY from the sample, fewer sites are left to bind IHCY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IHCY concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Total Homocystein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HCY and analogues was observed. | |
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Summary of the Assay Procedure for Rat Total Homocystein ELISA kit
Citations of Rat Total Homocystein ELISA kit
E02H0031 has been referenced in the below publications：
THE EFFECT OF EXCESSIVE INTAKE OF IODINE ON ACTIVALTION OF INFERIOR VENA CAVA ENDOTHELIAL CELLS OF RATS.
Effects of macrophage polarization during development of liver cirrhosis in rats.
