En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Total Homocystein ELISA kit (E02H0031)

E02H0031 Rat Total Homocystein ELISA kit

The Rat Total Homocystein ELISA kit can be used to identify samples from the rat species. Total Homocystein can also be called Homocysteine, Hcy, HCY.

Products

Specifications of Rat Total Homocystein ELISA kit

Product Information

Cat. No.

E02H0031

Product Name

Rat Total Homocystein ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5-100 μmol/L

Sensitivity

1.0 umol/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 umol/L

1 vial

STANDARD B (0.5mL)

50 umol/L

1 vial

STANDARD C (0.5mL)

100 umol/L

1 vial

STANDARD D (0.5mL)

250 umol/L

1 vial

STANDARD E (0.5mL)

500 umol/L

1 vial

STANDARD F (0.5mL)

1000 umol/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HCY ELISA kit uses an anti-IHCY antibody and an IHCY-HRP conjugate in a competitive enzyme immunoassay method. IHCY-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IHCY antibody binding site between IHCY from samples and IHCY-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IHCY. Since the number of sites is limited, as more sites are occupied by IHCY from the sample, fewer sites are left to bind IHCY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IHCY concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Total Homocystein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HCY and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Total Homocystein ELISA kit

Summary of the Assay Procedure for Rat Total Homocystein ELISA kit

Citations of Rat Total Homocystein ELISA kit

E02H0031 has been referenced in the below publications:

THE EFFECT OF EXCESSIVE INTAKE OF IODINE ON ACTIVALTION OF INFERIOR VENA CAVA ENDOTHELIAL CELLS OF RATS.

Effects of macrophage polarization during development of liver cirrhosis in rats.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.