E02T0058 Rat Transforming Growth Factor β ELISA kit
The Rat Transforming Growth Factor β ELISA kit can be used to identify samples from the rat species. Transforming Growth Factor β can also be called TGFβ.
Specifications of Rat Transforming Growth Factor β ELISA kit
Product Information | |
Cat. No. | E02T0058 |
Product Name | Rat Transforming Growth Factor β ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TGFβ ELISA kit uses an anti-TGFβ antibody and an TGFβ-HRP conjugate in a competitive enzyme immunoassay method. TGFβ-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TGFβ antibody binding site between TGFβ from samples and TGFβ-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TGFβ. Since the number of sites is limited, as more sites are occupied by TGFβ from the sample, fewer sites are left to bind TGFβ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFβ concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Transforming Growth Factor β ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TGFβ and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Transforming Growth Factor β ELISA kit
Citations of Rat Transforming Growth Factor β ELISA kit
E02T0058 has been referenced in the below publications：
The effects of JiaweiXiaokekang on the expressions of factors regulating the hepatic extracellular matrix in DN rats.
祖师麻肠溶制剂的药效学研究。
